rabbit anti human cdk6 mab  (Proteintech)

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: SYBR green qPCR primers used in this study.

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques: SYBR Green Assay, Sequencing

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: Antibodies used in western blot.

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques: Western Blot

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: Impact of miR‐29c‐3p on protein expression in PESC1 primary endometriotic stromal cells. The protein expression of CDK6 and PTEN is decreased, and p21 is increased in pre‐miR‐29c‐3p‐transfected primary endometrial stromal cells isolated from patient 1. Representative bands are shown in the left panel, and the quantitative analysis via densitometric scanning is shown in the right panel ( n = 3). In the CDK6 and corresponding tubulin panel, the original image was mirrored (left–right) to show a loading order that is consistent with the other figures of the publication, as described in methods. Data represent the mean ± SEM. Two‐tailed Student's t‐test (* p < 0.05). Data from primary endometrial stromal cells of patients 2 and 3 are shown in Figures and , respectively.

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques: Expressing, Transfection, Isolation, Two Tailed Test

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: miR‐29c‐3p diminishes the expression of several mRNAs in 12Z epithelial endometriotic cells (A) Bioinformatic analysis by the Target Scan algorithm identifies CDK6, COL4A2, TCF4 (TCF7L2), PTEN, and ROBO1 as predicted targets of miR‐29c‐3p. Shown is an alignment of targets predicted binding sites of the miR‐29c‐3p seed sequence to the respective target mRNAs. Alignment data are taken from the targetscan.org . (B) The fold change expression of CDK6, BCCIP, p21, PTEN, TCF7L1, TCF7L2, COL4A2, ROBO1, and vimentin is compared to negative control‐transfected cells (control). Data are presented as means ± SEM ( n = 3). Two tailed Student's t ‐test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques: Expressing, Binding Assay, Sequencing, Negative Control, Transfection, Control, Two Tailed Test

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: miR‐29c‐3p diminishes the expression of several mRNAs in primary endometriotic stromal cells. The fold change expression of CDK6 , BCCIP , p21 , PTEN , TCF7L1 , TCF7L2 , COL4A2 , ROBO1 , vimentin , E‐Cadherin ( CDH1 ), and N‐Cadherin ( CDH2 ) in pre‐miR‐29c‐3p‐transfected (A) primary endometriotic stromal cells isolated from patient 1 (PESC1), (B) primary endometriotic stromal cells isolated from patient 2 (PESC2), and (C) primary endometriotic stromal cells isolated from patient 3 (PESC3), compared to negative control‐transfected cells (control). Data are presented as means ± SEM ( n = 3). Two‐tailed Student's t ‐test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques: Expressing, Transfection, Isolation, Negative Control, Control, Two Tailed Test

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: Impact of miR‐29c‐3p on protein expression in 12Z endometriotic cells. The protein expression of CDK6, TCF7L2, and PTEN is decreased in pre‐miR‐29c‐3p‐transfected 12Z cells after 72 h of transfection, and p21 protein is increased compared to negative control‐transfected cells (control). Representative bands are shown in the left panel, and the quantitative analysis via densitometric scanning is shown in the right panel ( n = 3). Data represent the mean ± SEM. Two‐tailed Student's t‐test (* p < 0.05).

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques: Expressing, Transfection, Negative Control, Control, Two Tailed Test

Journal: Reproductive Medicine and Biology

Article Title: Combined targeting of TCF7L1 /2, PTEN , CDK6 , and BCCIP by microRNA miR ‐29c‐3p is associated with reduced invasion and proliferation of endometriotic cells

doi: 10.1002/rmb2.12645

Figure Lengend Snippet: Model of miR‐29c‐3p's impact on endometriotic cell proliferation and invasion. Upregulation of miR‐29c‐3p leads to the downregulation of cell cycle regulators, including CDK6 and BCCIP (an inhibitor of p21), resulting in the upregulation of p21. This, in turn, inhibits CDK6, significantly reducing cell proliferation. Additionally, miR‐29c‐3p downregulates epithelial–mesenchymal transition (EMT) regulators such as TCF7L1, TCF7L2, COL4A2, and N‐Cadherin (CDH2), potentially reducing EMT and thereby inhibiting invasion. Blue arrows indicate the mechanistic effects of miR‐29c‐3p. ↑ corresponds to upregulation/increase, and ↓ corresponds to downregulation/decrease. The figure was created using BioRender ( www.biorender.com ).

Article Snippet: Rabbit anti‐human CDK6 , Cell Signaling Technology, Danvers, MA, USA , 13331 , 1:1000.

Techniques:

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 6. CDK6 and EGFR are targets of miR-1296-5p (A) Schematic graph of wild-type and mutant putative binding sites of miR-1296-5p targeting the 3′-UTR of CDK6 and EGFR. (B) Relative luciferase activity of SGC-7901 and GSC-803 cells after cotransfection with wild-type or mutant CDK6 and EGFR 3′-UTR

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques: Mutagenesis, Binding Assay, Luciferase, Activity Assay, Cotransfection

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 7. EGFR and CDK6 are down-regulated in GC and negatively correlated with miR-1296-5p

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques:

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 8. Downregulation of EGFR and CDK6 are key to the antiproliferative function of miR-1296-5p (A) Representative Western blots for CDK6 and EGFR in SGC-7901 and GSC-803 cells. (B) Cell viability was examined by an MTT assay in SGC-7901 and GSC-803 cells. Bar graphs represent the mean +−SEM from at least three independent experiments;

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques: Western Blot, MTT Assay

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 9. Overexpression of EGFR and CDK6 reversed the inhibitory effect of miR-1296-5p on cell invasion Representative images of invasive cells on polycarbonate Transwell membranes. Bar graphs represent the average number of invasive cells per field from three independent experiments; *P<0.05, **P<0.01, and ***P<0.001 vs. the indicated groups.

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques: Over Expression